1. Who can I contact for more information?
  2. What is the distribution fee for a cryo-recovered litter or cryo-preserved germplasm?
  3. After I order my cryo-preserved sperm or embryos, how long before I receive them?
  4. After I order my cryo-recovered litter, how long before I receive the mice?
  5. Once I know which mouse strain I want, how do I order them?
  6. Where can I find more information on how cryo-preserved germplasm is shipped?
  7. Where can I find the protocols used for cryo-preservation and thawing?
  8. How many embryos or sperm will I receive for my order?
  9. When will I receive a health report if I have ordered a cryo-recovered litter?
  10. How should I note the mice in publication, and acknowledge the donating party and MMRRC?
  11. What form of payment do you accept?
 
Who can I contact for more information?
If you cannot find the answer to your question(s) here, you can always get more help by calling the MMRRC at UC Davis
at (530) 757-3287, sending a fax to (530) 757-3284, or sending an email to mmrrc@ucdavis.edu.
 
Please also review the Cryo-recovery and Distribution of Cryo-material pages.
 
What is the distribution fee for a cryo-recovered litter or cryo-preserved germplasm?
For the distribution fees for the MMRRC, please see: http://www.mmrrc.org/catalog/fees.php
 
Please note that there are additional handling and/or crate fees. Once the order is placed online, an order estimate will
be generated and emailed to you.
 
For shipping charges, we ask that you provide your own specialized courier account number to cover the shipment of
the recovered litter of mice or cryo-preserved germplasm.
 
After I order my cryo-preserved sperm or embryos, how long before I receive them?
Cryo-preserved sperm and embryos are ready to ship out as soon as we receive the online order, MTA/COU, and
payment. Actual shipment date depends on availability of a dry shipper and whether customs documentation is required
(for international orders only).
 
After I order my cryo-recovered litter, how long before I receive the mice?
Recovered litters are usually available to ship in 3 to 4 months after we receive the online order, MTA/COU, and
payment.
 
Please view our Resuscitation Timeline for more details.
 
Once I know which mouse strain I want, how do I order them?
To place an order, please see instructions at this url: http://www.mmrrc.org/catalog/requestform_helpdoc.php
 
This includes a link to our catalogue search at this url: http://www.mmrrc.org/catalog/StrainCatalogSearchForm.php
 
You can view general distribution information at this URL: http://www.mmrrc.org/catalog/distrInfo.php
 
If you need assistance with placing an order, please call the MMRRC customer service center at 1-800-910-2291 (North
America) or 530-757-5710 (International). You may also email them at service@mmrrc.org.
 
Where can I find more information on how cryo-preserved germplasm is shipped?
Cryo-preserved germplasm is shipped via FedEx (domestic) or specialized courier such as World Courier (international).
All samples are shipped in a SC4/2V nitrogen vapor shipper (dry shipper). Dry shippers are prepared for shipment by
filling with liquid nitrogen, which is absorbed into the lining. Liquid nitrogen not absorbed after 24 hours is poured off
before shipping. The shipper is called a “dry” shipper because it no longer contains a liquid. A properly prepared Dry
Shipper is not considered to be a Dangerous Good and is not restricted. It does not contain liquid nitrogen. It does
contain vaporous nitrogen.
 
Additional information on the SC4/2V dry shipper used by the MMRRC at UC Davis can be found at: https://www.chart-ind.com/litfiles/11550825.pdf
 
Dry shippers are quality controlled on a regular basis by MMRRC at UC Davis staff. A dry shipper in proper working order
will remain charged for at least 7 days, allowing for plenty of time for customs clearance or other delays, if any.
 
Where can I find the protocols used for cryo-preservation and thawing?
For cryo-preservation and thawing of embryos in plastic semen straws with propylene glycol, use the MCRL Embryo
Cryopreservation Protocol. Nearly all of the MMRRC at UC Davis frozen embryos which are shipped out use this
protocol.
 
For thawing embryos preserved in cryo-vials with DMSO, use the 8-Cell Slow Rate Cryo Thawing Method. A few of the
older preserved lines, and lines redistributed to us from the Taconic/Albany MMRRC used this method; however, we are
re-archiving these lines with the newer method above.
 
For cryo-preservation of mouse sperm, we use the Mouse Sperm Cryopreservation protocol.
 
For IVF using cryo-preserved mouse sperm, use the MCRL IVF Protocol.
 
How many embryos or sperm will I receive for my order?
One aliquot of embryos will contain 40-50 embryos in one or two straws. An aliquot contains a sufficient number of
embryos to transfer to at least two recipients.
One aliquot of sperm is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided
protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these
procedures when performed outside the MMRRC facilities. Neither gender nor genotype ratios are guaranteed.
 
When will I receive a health report if I have ordered a cryo-recovered litter?
For our maternity room (located in our M3 facility), there is no single report available as we do not have sentinels in that
room; instead all embryo recipients are screened. A report will be provided from the embryo recipients once the pups
are weaned and the recipients are screened. Testing and report preparation takes about 2 weeks post weaning and
submission.
 
The M3 facility is a shower-in barrier facility with autoclaved or gassed in supplies, including clothing. Staff wear mask,
hair bonnet, gloves. Mice are housed in Thoren caging systems with change stations. Entry is limited to approved
animal care and transgenic core personnel only; primary investigators and their technicians are not allowed in this
barrier facility. All mice are derived in by embryo transfer.
 
For more information on our barrier facility, including investigator, shipping contact, and campus veterinarian, please
see: Summary of M3 Practices
 
How should I note the mice in publication, and acknowledge the donating party and MMRRC?
The mouse strain used for this research project, (insert full strain name), identification number 0#####-UCD, was
obtained from the Mutant Mouse Regional Resource Center, a NIH funded strain repository, and was donated to the
MMRRC by (the NHLBI funded BayGenomics project OR the NINDS funded GENSAT BAC transgenic project OR Lexicon
Genetics Incorporated OR Genentech, Inc. OR insert donating investigator).
 
Please contact us at mmrrc@ucdavis.edu for any questions regarding acknowledgement for MMRRC strains. We also
encourage you to contact us when you have published using an MMRRC strain.
 
What form of payment do you accept?
For domestic customers we accept purchase orders (PO), credit card, check, and wire transfers. For international
customers we accept only credit card, check, and wire transfers. We do require payment prior to processing your
order. Please visit our payment information page for additional information and payment forms.