DEXA for Project: MAS225N1 Test Description: Mice were anesthetized by intraperitoneal injection of Avertin (1.25% 2,2,2,-tribromoethanol, 20 ml/kg body weight ), measured body length and weight, and then placed in a prone position on the platform of the PIXImusTM Densitometer (Lunar Inc.) for a DEXA scan. Using a Lunar PIXImus software, the Bone mineral density (BMD) and fat composition (% fat) and total tissue mass (TTM) were determined in the regions of interest (ROI, i.e., whole body, vertebrae, and both femurs).

Level I   BMD = Bone Mineral Density (g/cm2);   BMC = Bone Mineral Content (g);   LBM = Lean Body Mass (g);   BMC / LBM = BMC / LBM (mg/g);   FAT(%) = % of Body FAT;   FAT(g) = TTM X FAT(%)/100;   TTM = Total Tissue Mass (g);   vBMD = Volumetric BMD (mg/cm3) MALE DATA FEMALE DATA DEXA Combined Gender Score Parameter Score Body BMD  96.8% Body vBMD  98.5% Body BMC/LBM  100.7% Femur BMD  96.4% Vertebral BMD  96.1% Total Tissue Mass  90.6% Body LBM  92.8% Body % Fat  88.4% Body Fat Content  81.0% The Combined Gender Score provides the average ration of Hom to WT values combining males and females, with a correction factor for the number of mice in each group. No score is generated if there are fewer than 4 HOM mice or fewer than 3 WT mice

DEXA Analysis: Male Mouse Label Genotype Whole Body Vertebrae Femurs BMD (g/cm2) BMC (g) TTM % FAT LBM (g) BMC / LBM (mg/g) vBMD (mg/cm3) BMD (g/cm2) BMD (g/cm2) MAS225N1-M-61 wt 0.0509 0.5723 30.34 12.09 26.1 21.93 15.18 0.0542 0.07905000 MAS225N1-M-93 wt 0.0606 0.6639 36.06 21.35 27.7 23.97 18.3 0.0626 0.09900001 MAS225N1-M-50 het 0.0501 0.5173 33.97 22.78 25.71 20.12 15.59 0.0533 0.07945000 MAS225N1-M-68 het 0.0542 0.5266 30.93 20.99 23.91 22.02 17.39 0.0523 0.07540000 MAS225N1-M-106 hom 0.0564 0.5397 35.85 22.43 27.27 19.79 18.24 0.0669 0.08800000 MAS225N1-M-123 hom 0.0472 0.4943 26.61 9.81 23.51 21.03 14.58 0.0493 0.07225000 MAS225N1-M-81 hom 0.0551 0.5699 29.27 17.51 23.57 24.17 17.13 0.0634 0.08575000 MAS225N1-M-92 hom 0.0486 0.4806 27.77 10.9 24.26 19.81 15.45 0.0512 0.08115000

DEXA Analysis: Female Mouse Label Genotype Whole Body Vertebrae Femurs BMD (g/cm2) BMC (g) TTM % FAT LBM (g) BMC / LBM (mg/g) vBMD (mg/cm3) BMD (g/cm2) BMD (g/cm2) MAS225N1-F-111 wt 0.0493 0.4529 20.05 18.11 15.97 28.37 16.26 0.0642 0.07135000 MAS225N1-F-117 wt 0.0508 0.4498 25.73 22.07 19.6 22.95 17.07 0.0583 0.07435000 MAS225N1-F-110 het 0.0449 0.3894 18.26 16.2 14.91 26.11 15.26 0.0509 0.06545000 MAS225N1-F-124 het 0.0483 0.4665 19.49 15.29 16.04 29.08 15.54 0.0583 0.06710000 MAS225N1-F-52 hom 0.053 0.5053 24.0 16.73 19.48 25.94 17.16 0.0638 0.07910000 MAS225N1-F-66 hom 0.0471 0.4379 17.21 12.83 14.56 30.07 15.44 0.0515 0.06785000 MAS225N1-F-75 hom 0.049 0.4374 18.81 16.71 15.23 28.72 16.4 0.0556 0.06985000 MAS225N1-F-95 hom 0.0522 0.4786 23.51 23.01 17.62 27.16 17.25 0.0581 0.07715000

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