DEXA for Project: SEC437N1 Test Description: Mice were anesthetized by intraperitoneal injection of Avertin (1.25% 2,2,2,-tribromoethanol, 20 ml/kg body weight ), measured body length and weight, and then placed in a prone position on the platform of the PIXImusTM Densitometer (Lunar Inc.) for a DEXA scan. Using a Lunar PIXImus software, the Bone mineral density (BMD) and fat composition (% fat) and total tissue mass (TTM) were determined in the regions of interest (ROI, i.e., whole body, vertebrae, and both femurs).

Level I   BMD = Bone Mineral Density (g/cm2);   BMC = Bone Mineral Content (g);   LBM = Lean Body Mass (g);   BMC / LBM = BMC / LBM (mg/g);   FAT(%) = % of Body FAT;   FAT(g) = TTM X FAT(%)/100;   TTM = Total Tissue Mass (g);   vBMD = Volumetric BMD (mg/cm3) MALE DATA FEMALE DATA DEXA Combined Gender Score Parameter Score Body BMD  95.4% Body vBMD  96.6% Body BMC/LBM  102.4% Femur BMD  96.8% Vertebral BMD  92.1% Total Tissue Mass  86.8% Body LBM  90.7% Body % Fat  85.0% Body Fat Content  73.3% The Combined Gender Score provides the average ration of Hom to WT values combining males and females, with a correction factor for the number of mice in each group. No score is generated if there are fewer than 4 HOM mice or fewer than 3 WT mice

DEXA Analysis: Male Mouse Label Genotype Whole Body Vertebrae Femurs BMD (g/cm2) BMC (g) TTM % FAT LBM (g) BMC / LBM (mg/g) vBMD (mg/cm3) BMD (g/cm2) BMD (g/cm2) SEC437N1-M-126 wt 0.0608 0.6578 33.75 19.28 26.59 24.74 18.48 0.0772 0.10400000 SEC437N1-M-89 wt 0.0615 0.6393 40.8 24.52 30.16 21.2 19.07 0.0737 0.10155000 SEC437N1-M-140 het 0.0541 0.5508 30.76 19.16 24.32 22.65 16.96 0.0594 0.08795001 SEC437N1-M-88 het 0.0624 0.7347 40.46 18.11 32.4 22.68 18.19 0.0684 0.09580000 SEC437N1-M-108 hom 0.0608 0.6206 32.47 18.87 25.72 24.13 19.03 0.0741 0.10245000 SEC437N1-M-119 hom 0.0546 0.5607 32.49 18.7 25.85 21.69 17.04 0.057 0.09150000 SEC437N1-M-121 hom 0.0545 0.61 26.53 11.62 22.84 26.71 16.28 0.0653 0.08330000 SEC437N1-M-94 hom 0.0545 0.5627 35.33 21.87 27.04 20.81 16.97 0.0603 0.08555000

DEXA Analysis: Female Mouse Label Genotype Whole Body Vertebrae Femurs BMD (g/cm2) BMC (g) TTM % FAT LBM (g) BMC / LBM (mg/g) vBMD (mg/cm3) BMD (g/cm2) BMD (g/cm2) SEC437N1-F-104 wt 0.055 0.5139 29.93 28.85 20.78 24.73 17.99 0.0629 0.07830000 SEC437N1-F-134 wt 0.0532 0.5411 24.45 17.33 19.67 27.51 16.68 0.0676 0.07980000 SEC437N1-F-100 het 0.0487 0.4944 24.48 20.57 18.95 26.09 15.29 0.0568 0.07240000 SEC437N1-F-160 het 0.0506 0.4643 21.73 21.59 16.57 28.01 16.7 0.061 0.07755000 SEC437N1-F-143 hom 0.056 0.5405 24.11 18.22 19.18 28.19 18.03 0.0684 0.08625000 SEC437N1-F-145 hom 0.0491 0.4397 21.35 20.15 16.61 26.47 16.4 0.0533 0.07360000 SEC437N1-F-153 hom 0.0571 0.5619 26.51 21.47 20.26 27.74 18.2 0.0714 0.09015000 SEC437N1-F-98 hom 0.052 0.4677 24.24 22.34 18.36 25.48 17.33 0.0656 0.08305000

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