DEXA for Project: SEC468N1 Test Description: Mice were anesthetized by intraperitoneal injection of Avertin (1.25% 2,2,2,-tribromoethanol, 20 ml/kg body weight ), measured body length and weight, and then placed in a prone position on the platform of the PIXImusTM Densitometer (Lunar Inc.) for a DEXA scan. Using a Lunar PIXImus software, the Bone mineral density (BMD) and fat composition (% fat) and total tissue mass (TTM) were determined in the regions of interest (ROI, i.e., whole body, vertebrae, and both femurs).

Level I   BMD = Bone Mineral Density (g/cm2);   BMC = Bone Mineral Content (g);   LBM = Lean Body Mass (g);   BMC / LBM = BMC / LBM (mg/g);   FAT(%) = % of Body FAT;   FAT(g) = TTM X FAT(%)/100;   TTM = Total Tissue Mass (g);   vBMD = Volumetric BMD (mg/cm3) MALE DATA FEMALE DATA DEXA Combined Gender Score Parameter Score Body BMD  96.5% Body vBMD  97.2% Body BMC/LBM  97.6% Femur BMD  93.5% Vertebral BMD  92.9% Total Tissue Mass  101.6% Body LBM  97.6% Body % Fat  116.2% Body Fat Content  117.6% The Combined Gender Score provides the average ration of Hom to WT values combining males and females, with a correction factor for the number of mice in each group. No score is generated if there are fewer than 4 HOM mice or fewer than 3 WT mice

DEXA Analysis: Male Mouse Label Genotype Whole Body Vertebrae Femurs BMD (g/cm2) BMC (g) TTM % FAT LBM (g) BMC / LBM (mg/g) vBMD (mg/cm3) BMD (g/cm2) BMD (g/cm2) SEC468N1-M-144 wt 0.0579 0.657 29.08 12.86 24.68 26.62 17.19 0.0713 0.09440000 SEC468N1-M-67 wt 0.0571 0.5608 33.42 23.21 25.1 22.34 18.22 0.0613 0.09155000 SEC468N1-M-101 het 0.0518 0.5561 27.44 14.58 22.88 24.3 15.81 0.0576 0.07645001 SEC468N1-M-71 het 0.0592 0.6467 33.46 21.14 25.74 25.12 17.91 0.0701 0.09470000 SEC468N1-M-135 hom 0.0519 0.5448 31.61 18.33 25.27 21.56 16.02 0.0609 0.08005001 SEC468N1-M-141 hom 0.0575 0.635 33.15 15.92 27.24 23.31 17.31 0.0686 0.08885000 SEC468N1-M-155 hom 0.0572 0.6137 32.72 25.42 23.79 25.8 17.45 0.0682 0.09340000 SEC468N1-M-77 hom 0.0507 0.4728 26.5 15.8 21.84 21.65 16.61 0.056 0.07750000

DEXA Analysis: Female Mouse Label Genotype Whole Body Vertebrae Femurs BMD (g/cm2) BMC (g) TTM % FAT LBM (g) BMC / LBM (mg/g) vBMD (mg/cm3) BMD (g/cm2) BMD (g/cm2) SEC468N1-F-69 wt 0.0501 0.4305 24.52 25.65 17.8 24.19 17.08 0.064 0.07660000 SEC468N1-F-84 wt 0.0529 0.5125 24.29 14.73 20.2 25.37 16.99 0.0657 0.07840000 SEC468N1-F-130 het 0.0534 0.4708 24.4 22.11 18.53 25.4 17.98 0.0712 0.07755000 SEC468N1-F-187 het 0.051 0.4177 27.97 33.86 18.08 23.1 17.82 0.0658 0.07375000 SEC468N1-F-110 hom 0.0509 0.4631 25.99 25.69 18.85 24.57 16.87 0.0566 0.07760000 SEC468N1-F-153 hom 0.0499 0.4306 25.04 29.56 17.21 25.02 17.0 0.0591 0.06650000 SEC468N1-F-160 hom 0.0516 0.4903 24.82 22.47 18.75 26.15 16.73 0.0637 0.07680000 SEC468N1-F-76 hom 0.0507 0.452 25.61 25.46 18.64 24.25 16.99 0.0544 0.07535000

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