DEXA for Project: TRA267N1 Test Description: Mice were anesthetized by intraperitoneal injection of Avertin (1.25% 2,2,2,-tribromoethanol, 20 ml/kg body weight ), measured body length and weight, and then placed in a prone position on the platform of the PIXImusTM Densitometer (Lunar Inc.) for a DEXA scan. Using a Lunar PIXImus software, the Bone mineral density (BMD) and fat composition (% fat) and total tissue mass (TTM) were determined in the regions of interest (ROI, i.e., whole body, vertebrae, and both femurs).

Level I   BMD = Bone Mineral Density (g/cm2);   BMC = Bone Mineral Content (g);   LBM = Lean Body Mass (g);   BMC / LBM = BMC / LBM (mg/g);   FAT(%) = % of Body FAT;   FAT(g) = TTM X FAT(%)/100;   TTM = Total Tissue Mass (g);   vBMD = Volumetric BMD (mg/cm3) MALE DATA FEMALE DATA DEXA Combined Gender Score Parameter Score Body BMD  N/A Body vBMD  N/A Body BMC/LBM  N/A Femur BMD  N/A Vertebral BMD  N/A Total Tissue Mass  N/A Body LBM  N/A Body % Fat  N/A Body Fat Content  N/A The Combined Gender Score provides the average ration of Hom to WT values combining males and females, with a correction factor for the number of mice in each group. No score is generated if there are fewer than 4 HOM mice or fewer than 3 WT mice

DEXA Analysis: Male Mouse Label Genotype Whole Body Vertebrae Femurs BMD (g/cm2) BMC (g) TTM % FAT LBM (g) BMC / LBM (mg/g) vBMD (mg/cm3) BMD (g/cm2) BMD (g/cm2) TRA267N1-M-114 wt 0.0553 0.548 30.06 18.77 23.87 22.96 17.58 0.0598 0.08720000 TRA267N1-M-131 wt 0.0522 0.5217 28.49 17.06 23.11 22.58 16.51 0.0588 0.08419999 TRA267N1-M-111 het 0.0533 0.5015 30.3 17.95 24.36 20.59 17.38 0.0596 0.07845000 TRA267N1-M-116 het 0.0498 0.4566 32.42 20.13 25.44 17.95 16.45 0.0546 0.08205000 TRA267N1-M-125 het 0.0533 0.5475 29.97 13.7 25.32 21.63 16.63 0.0633 0.08765000 TRA267N1-M-130 het 0.0541 0.5297 27.47 12.69 23.45 22.58 17.28 0.0603 0.08825000

DEXA Analysis: Female Mouse Label Genotype Whole Body Vertebrae Femurs BMD (g/cm2) BMC (g) TTM % FAT LBM (g) BMC / LBM (mg/g) vBMD (mg/cm3) BMD (g/cm2) BMD (g/cm2) TRA267N1-F-122 wt 0.0434 0.3503 21.07 22.88 15.9 22.03 15.28 0.0536 0.06065000 TRA267N1-F-137 wt 0.0489 0.4555 19.96 14.32 16.65 27.36 16.03 0.0576 0.07105000 TRA267N1-F-117 het 0.0465 0.4184 21.05 18.11 16.82 24.88 15.51 0.056 0.06515000 TRA267N1-F-123 het 0.0465 0.3936 21.5 22.35 16.3 24.15 15.99 0.0523 0.06850000 TRA267N1-F-129 het 0.0522 0.4928 23.47 18.23 18.7 26.35 16.99 0.0673 0.07645001 TRA267N1-F-142 het 0.0471 0.3954 23.72 26.92 16.94 23.34 16.25 0.0525 0.06660000

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