Are you looking for Cre or FLP deleter lines that are on C57BL/6N background? Look no further, the NIH supported Mutant Mouse Regional Resource Centers (MMRRC) distribute two such lines, which are currently available as LIVE MICE:
034412-UCD C57BL/6N-Hprttm1(CMV-cre)Brd/Mmucd – This model, donated by Allan Bradley and the Wellcome Trust Sanger Institute, is an X-linked Cre-deleter line which utilizes the CMV promoter to drive the Cre recombinase activity. The Hprttm1(CMV-cre)Brd construct was electroporated into JM8.F6 ES cells and the strain has been maintained on C57BL/6NTac.
036512-UCD C57BL/6N-Tg(CAG-Flpo)1Afst/Mmucd – This model, originally donated by Konstantinos Anastassiadis and Technische Universitaet Dresden, has the optimized FLP recombinase which utilizes CAG promoter to drive the FLPo activity. This strain was created using JM8.F6 ES cells, mice were mated to albino C57BL/6J, and then subsequently backcrossed back onto C57BL/6NTac by the UC Davis Mouse Biology Program and donated to the MMRRC.
Other Cre and FLP lines can also be found at the MMRRC, check them out at www.mmrrc.org, you can search by promoter, recombinase, or MGI number.
Questions on these or other MMRRC mouse models? Please contact service@mmrrc.org, and the customer service team will be happy to assist.
Updated on April 9th, 2012The MMRRC accepts new strains and cell lines frequently to fulfill the scientific community's needs. Please visit the monthly reports page for the list of newest available/accepted strains.
mmrrc@ucdavis.edu
Phone: (530) 754-MMRRC(6677)
Fax: (530) 757-3284
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