Author: Jason Aller

Mouse Biology Program is in the process of an across the board rate review, affecting MBP, MMRRC at UC Davis, MMPC at UC Davis, GMRC, and MBSR. Our rates will be updated as early as October 1, 2023. Orders submitted prior to that date will not be affected, however fees quoted but no order request submitted will be subject to the new rates. Our per diem rates for all MBP housed and managed colonies will be billed the new rates starting October 23^rd. We will release a copy of the new rates as soon as they are approved. 

The IMPC Strategy Report for 2021-2030 entitled “The Function of Human Genetic Variation” has been released.

The MMRRC at UC Davis will be implementing some fee changes to our ES Cell lines and associated services.  We will be performing genetic quality control on all cell lines prior to order fulfillment, thus incorporating that cost to the clone fees. The new pricing will be posted and take effect on February 11, 2019, coinciding with the import of the KOMP ES Cell lines to the MMRRC for distribution. We will honor previously quoted rates for current projects with ongoing services, including new projects quoted and initiated prior to February 11, 2019. Projects initiated after February 11, or any newly requested add-on services to existing projects, will be quoted and billed the new approved rate(s).

For more information about the MMRRC at UCD’s rates please email mmrrc@ucdavis.edu.

Are you looking for a FLP deleter lines that is on a C57BL/6N background?  Look no further, the NIH supported Mutant Mouse Regional Resource Centers (MMRRC) distributes such a line, which is currently available as LIVE MICE:

036512-UCD C57BL/6N-Tg(CAG-Flpo)1Afst/Mmucd – This model, originally donated by Konstantinos Anastassiadis and Technische Universitaet Dresden, has the optimized FLP recombinase which utilizes CAG promoter to drive the FLPo activity. This strain was created using JM8.F6 ES cells, mice were mated to albino C57BL/6J, and then subsequently backcrossed back onto C57BL/6NTac by the UC Davis Mouse Biology Program and donated to the MMRRC.

Other Cre and FLP lines can also be found at the MMRRC, check them out at www.mmrrc.org, you can search by promoter, recombinase, or MGI number.  

Questions on these or other MMRRC mouse models? Please contact service@mmrrc.org, and the customer service team will be happy to assist.

The Mutant Mouse Resource and Research Center (MMRRC), the official National Institute of Health (NIH) repository of mouse models, is pleased to announce the availability of genetically-altered mice and embryonic stem (ES) cells made as part of the NIH Knockout Mouse Project (KOMP) and previously maintained in the KOMP Repository. The KOMP Repository collection will provide investigators with the convenience of a one-stop portal to one of the largest inventories of mutant mouse strains and ES cell lines available to the biomedical research community.

These newly acquired mouse and ES cell lines have been deposited into the MMRRC at UC Davis. The MMRRC at UC Davis is the largest of four regional archive and distribution centers in the NIH consortium. The MMRRC functions as a single repository resource and is comprised of an Informatics, Coordination and Service Center (ICSC) and three additional regional distribution facilities which include: The Jackson Laboratory, University of Missouri, and University of North Carolina, Chapel Hill. The newly available KOMP Repository mice (4,175 unique lines) & ES cell lines (14,013 unique mutant lines and 7 parental lines) can be accessed by visiting the MMRRC website (www.mmrrc.org) and typing in “KOMP Repository” in the search function, or by using the advanced search function and indicating “Major Collection = KOMP”, and then searching by gene of interest, which will allow filtering for ES cells or mice.

The MMRRC was created in 1999, and is supported through the NIH, Office of Infrastructure and Research Programs (ORIP), as the nation’s premier mouse archive and distribution repository. Since that time, the MMRRC has earned an international reputation for the management, cryopreservation, and distribution of scientifically valuable, genetically engineered mouse strains and mouse ES cells. In partnership with researchers around the globe, the MMRRC continues to expand its holdings of mouse models. Today, with more than 59,000 available models, the MMRRC serves as a valuable resource to drive research discoveries for human disease.

2019-02-20 The Mutant Mouse Resource and Research Center (MMRRC), the official National Institute of Health (NIH) repository of mouse models, is pleased to announce the availability of genetically-altered mice and embryonic stem (ES) cells made as part of the NIH Knockout Mouse Project (KOMP) and previously maintained in the KOMP Repository. The KOMP Repository collection will provide investigators with the convenience of a one-stop portal to one of the largest inventories of mutant mouse strains and ES cell lines available to the biomedical research community.

These newly acquired mouse and ES cell lines have been deposited into the MMRRC at UC Davis. The MMRRC at UC Davis is the largest of four regional archive and distribution centers in the NIH consortium. The MMRRC functions as a single repository resource and is comprised of an Informatics, Coordination and Service Center (ICSC) and three additional regional distribution facilities which include: The Jackson Laboratory, University of Missouri, and University of North Carolina, Chapel Hill. The newly available KOMP Repository mice (4,175 unique lines) & ES cell lines (14,013 unique mutant lines and 7 parental lines) can be accessed by visiting the MMRRC website (www.mmrrc.org) and typing in “KOMP Repository” in the search function, or by using the advanced search function and indicating “Major Collection = KOMP”, and then searching by gene of interest, which will allow filtering for ES cells or mice.

The MMRRC was created in 1999, and is supported through the NIH, Office of Infrastructure and Research Programs (ORIP), as the nation’s premier mouse archive and distribution repository. Since that time, the MMRRC has earned an international reputation for the management, cryopreservation, and distribution of scientifically valuable, genetically engineered mouse strains and mouse ES cells. In partnership with researchers around the globe, the MMRRC continues to expand its holdings of mouse models. Today, with more than 59,000 available models, the MMRRC serves as a valuable resource to drive research discoveries for human disease.

Arjan Pol, et. al. : Mutations in SELENBP1, encoding a novel human methanethiol oxidase, cause extraoral halitosis Nature Genetics, JAN 2018; V50; 120-129

2017-01-12 from Nature News:  Oliver Smithies, a pioneer in the field of gene targeting, has died aged 91. Smithies shared the 2007 Nobel prize for medicine for a technique that allowed biologists to easily identify the function of genes, by generating ‘knockout’ mice — mutants in which specific genes are disabled. Smithies was a professor at the University of North Carolina at Chapel Hill. https://www.nytimes.com/2017/01/11/science/oliver-smithies-genetics-nobel-winner-dies-at-91.html?_r=0

2016-11-02 The IMPC is creating knockout mouse lines for every single protein coding gene in the mouse genome, and characterising them through standardised, quality-controlled phenotyping tests.

See the phenotypes for thousands of gene knockouts. Free database includes raw data, statistics, images, disease associations and interactive embryo viewer.

2016-10-20 The KOMP Repository has recently identified the presence of a translocation of the manipulated agouti allele on chromosome 8 of ES cell line JM8A3, and potentially affecting the sub-lines JM8A3.N1 and JM8A1.N3, that were all derived at the Wellcome Trust Sanger Institute. At this time, we recommended not to use these cells for targeting genes on chromosome 8.  Other lines, including, the JM8, JM8.N4, or JM8.F6 , do not exhibit this translocation and can be safely and reliably used for gene targeting experiments. If you are using mouse lines (e.g., KOMP targeted lines) derived from JM8A3, JM8A3.N1 or JM8A1.N3 ES cell lines, we recommend crossing to C57BL/6N wild type mice in order to breed out the agouti coat color, including chromosome 8 targeted alleles which may or may not be closely linked.  For further information or assistance please contact Allan Bradley at The Sanger Institute abradley@sanger.ac.uk.