ES cell thawing, culturing, and microinjection procedures differ depending on the parental cell line. To ensure best possible results, please be sure to use the appropriate protocol. These protocols are also available on the individual strain detail pages for each respective ES cell line (mutant and parental).
- Culturing and Media Conditions – JM8.N4
- Culturing and Media Conditions – JM8, JM8.F6
- Culturing and Media Conditions – JM8A sublines
- Culturing and Media Conditions – VGB6
- Culturing and Media Conditions – E14TG2a.4
- Culturing and Media Conditions – Soriano (AK7.1)
- Culturing and Media Conditions – Lex1
- Culturing and Media Conditions – Izon
- Culturing and Media Conditions – EAP1
- Culturing and Media Conditions – Bruce4
- Culturing and Media Conditions – BayGenomics
- Chromosome Spread and Counting
- Microinjection of Blastocysts – JM8, VGB6
- Microinjection of Blastocysts – JM8A
- Microinjection of Morulae – VGB6
- Microinjection – BayGenomics
- Electroporation
- Electroporation with FlpE plasmid
- Electroporation with Cre plasmid
- Electroporation – BayGenomics